TABLE 4.

Presence of HupB box in the promoter region of HupB-regulated genes

Misa	Gene	Locus no.	HupB boxb	Locc	Gene productd
0	mbtB	Rv2383c	CACTAAAATT	−40	Phenyloxazoline synthase
1	ptrBa	Rv0781	AACTAAAATT	−34	Protease oligopeptidase b
1	rv1578c	Rv1578c	GACTAAAAAT	−24	phiRv1 phage protein
2	hupBe	Rv2986c	CAGTGAAATT	−99	HupB 28–kDa iron–regulated protein (11)
2	rv1733c	Rv1733c	CAATAAAACT	−121	Conserved transmembrane protein
3	mbtA	Rv2384	CCCTAATTTT	−72	Salicyloyl-AMP ligase
3	mmpS5	Rv0677c	CTCTGAAATC	−71	Conserved membrane protein
3	rv0678	Rv0678	CAGTGAAACT	−11	Conserved hypothetical protein
3	rv3468c	Rv3468c	CGCGAAAAGT	−76	Glucose 4,6-dehydratase
3	papA3	Rv1182	CACAAAGATC	−131	Polyketide synthase protein
3	ppe63	Rv3539	CGCTAAAGGT	−124	PPE family protein
3	rv1730c	Rv1730c	CAACCAAATT	−210	Penicillin-binding protein
4	rv0368c	Rv0368c	CGCTAGAAGC	−190	Conserved hypothetical protein
4	phyA	Rv3397c	CACCGTAGTT	−20	Phytoene synthase
5	rv2087	Rv2087	CACCGATGCT	−5	Conserved hypothetical protein
5	bfrB	Rv3841	TATTATCATC	−91	Bacterioferritin
5	esxR	Rv3019c	CGCCAAGGTC	−143	Secreted ESAT-6-like protein

^aMismatch with the 10-bp HupB box (HupB-binding motif) in the mbtB promoter DNA experimentally proved in this study.

^bPutative HupB boxes (5′ to 3′).

^cLocation of putative HupB box with reference to the start site.

^dGene products are indicated as described by Mycobacterial Browser (Mycobrowser; Tuberculist version 2.6; http://tuberculist.epfl.ch/), except that the terms “probable” and “possible” are removed.

^eThis was used to prove the functionality of the HupB box in this study (Fig. 7B).