FIG 4.

Subcellular localization of P42 and the F₁-ATPase α subunit homolog, MMOB1660. Localizations of P42, MMOB1660, Gli349, DNA, and MvspI were examined by fluorescence microscopy. P42 and MMOB1660 were detected as EYFP fusion proteins. Gli349 and MvspI were detected by immunostaining, and DNA was detected by DAPI staining. Phase-contrast and fluorescence images were merged in the two panels at right. In merged images, a single cell in the left panel is magnified in the right panel. The transposon insertion sites were mapped at nucleotide 374965 on the minus strand and at nucleotide 187879 on the plus strand on the genome, respectively, for P42 and MMOB1660 localization in these images (see Table S1 in the supplemental material).