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OMTs are produced in the presence of metabolic inhibitors and in starvation buffer (TPM). (A) Cells harvested directly from a CTT plate, vortexed and centrifuged contain few OMTs (0 h; DW1047). Static incubation in the indicated media induces OMT production. Spectinomycin (Spec; 150 μg/ml) was added to 1/2× CTT. (B) OMTs in the presence of CCCP (25 μg/ml; strain DW1165). Bar, 8 μm.
