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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Antonie Van Leeuwenhoek. 2014 Feb 1;105(3):579–592. doi: 10.1007/s10482-014-0113-8

Fig. 4. Measurements of immunomodulatory properties are diminished in 6475eriC-Stop compared to WT 6475.

Fig. 4

(A) L. reuteri supernatants were tested for the ability to inhibit TNF by TLR2-activated THP-1 cells. Inhibition of TNF production by recombineered 6475eriC-Stop mutant was significantly reduced when compared to WT 6475. The results are expressed as the mean ± SD, n=3, *p-value < 0.05 compared to WT 6475 using unpaired t-test (B) L. reuteri-derived histamine was quantitated by a histamine-specific ELISA and demonstrated decreased histamine production in 6475eriC-Stop compared to WT 6475 even when grown in histidine-supplemented medium. Data were normalized to final culture OD600 = 1.0 and analyzed by 2-way ANOVA. Results represent the mean ± SD (n = 3), p value < 0.0001 compared to wild-type 6475.