Figure 1. NLRC3 attenuates DNA- and HSV-1-induced cytokines.

(A–B) WT and Nlrc3^−/− BMDMs were transfected (intracellular) or incubated (extracellular) with poly(dA:dT), transfected with poly(I:C) or treated with LPS. IFN-β and IL-6 were measured 16 hours after treatment. (C–D) Nlrc3^+/+ and Nlrc3^−/− MEFs were transfected with ISD and Ifna4 and Ifnb transcripts or (E) IFN-β measured 6 hours post-transfection. HSV-1 was used at MOI 0.05, 0.1 and 1 for panels F-J unless specified. (F–G) BMDMs were infected with HSV-1 and Ifna4 and Ifnb1 transcripts were measured. (H–J) Peritoneal macrophages were infected with HSV-1 and Ifna4, Ifnb1 and Tnf transcripts were measured. (K) Peritoneal macrophages were infected with HSV-1 (MOI 1) or SeV (80 HA unit/ml) and TNF assayed. (L–M) Primary MEFs were infected with HSV-1, and Ifnb1 and Tnf transcripts were measured. (N–O) Primary MEFs were infected with HSV-1 and IFNβ and IL-6 proteins were measured. (P) Primary MEFs or (Q) BMDMs were infected with HSV-1, genomic DNA was extracted and HSV relative genome copy number was determined by real-time PCR. All cytokines were determined by ELISA and transcripts determined by real-time PCR. All data are representative of at least three independent experiments.

See also Figure S1–S3