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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: Immunity. 2014 Feb 20;40(3):329–341. doi: 10.1016/j.immuni.2014.01.010

Figure 3. NLRC3 suppresses STING-TBK1-mediated IFN-β, ISRE and NF-κB reporter activation.

Figure 3

(A–D) HEK293T cells were transfected with the IFN-β, ISRE or NF-κB promoter reporter with the internal control Renilla luciferase reporter pLR-TK and empty vector or NLRC3 plasmid as indicated. Luciferase assays were performed 24 hours post transfection. (E) HEK293T cells were transfected with the ISRE promoter reporter with the internal control Renilla luciferase reporter pLR-TK. Stimulatory ligands were transfected 24 hours post plasmids transfection. Luciferase assays were performed 16 hours post ligand stimulation. (F) Same as A–D, except cells were transfected with empty vector or NLRC5. Data are representative of at least two independent experiments.