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. Author manuscript; available in PMC: 2014 May 6.
Published in final edited form as: Nat Immunol. 2010 Aug 8;11(9):862–871. doi: 10.1038/ni.1917

Table 1.

Expression profiles of key T cell lineage transcription factors in CD46-induced effector (TH1) and suppressor (Treg) cell populations

Transcription factor IFN-γ+IL-10−(TH1) IFN-γ+IL-10+ (Treg) IFN-γ−IL-10+ (Treg) TH1a TH2a
T-bet +++ +++ +++ ++++ −
GATA-3b −/+ + + −/+ ++++
Foxp3 − − − − −
p-STAT4 ++ +++ ++ ++++ ++
p-STAT5 +++ +++ + ++++ ++
p-STAT6 + + −/+ + ++++
p-Erk ++ +++ +++ ++++ ++
ICER/CREM +++ ++ + ++ ++

IL-10- and IFN-γ-secretion assays plus intracellular flow cytometry staining for T cell lineage markers in purified CD4+ T cells activated for 36 h with anti-CD3 and anti-CD46 in a high concentration of IL-2; transcription factor expression is presented relative to the expression in TH1 or TH2 cells as follows: ++++, highest; +++, strong; ++, moderate; +, low; −/+, weak but detectable; −, undetectable; p-, phosphorylated.

a

Purity of TH1 and TH2 cells assessed as percent IFN-γ+IL-4- cells (TH1) or IFN-γ−IL-4+ cells (TH2) after restimulation with anti-CD3 and anti-CD28 (>85% in all assays).

b

GATA-3 mRNA assessed by quantitative real-time RT-PCR analysis because of the lack of a suitable antibody.