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. Author manuscript; available in PMC: 2015 Apr 10.
Published in final edited form as: Mol Cell. 2014 Mar 20;54(1):119–132. doi: 10.1016/j.molcel.2014.02.020

Figure 1. IP6K2 binds to the TTT complex.

Figure 1

(A) SDS-PAGE of tandem-affinity purified IP6Ks, followed by silver-staining. Lower panel, western-blot of tandem-affinity purified IP6Ks identifies binding partner. (B) Co-IP between GST-IP6K2 and Flag-Tti1 expressed in HEK293 cells. (C) Co-IP between GST-IP6K2 and Flag-Tel2 expressed in HEK293 cells. (D) Endogenous Co-IP between IP6K2 and Tel2/Tti1 in HCT116 cells. (E) Co-IP between GST-IP6K2 fragments and Flag-Tti1. (F) Co-IP between GST-IP6K2 fragments and Flag-Tel2. (G) Direct binding between recombinant GST-IP6K2212-426 and purified Flag-Tti1 in vitro. (H) Co-IP between myc-Tti1 N (aa1-460), M (aa 461-825), and C (aa 826-1089) fragments and GST-IP6K2. (I) Direct binding between purified recombinant full-length IP6K2 and recombinant GST-Tti1MC (aa 461-1089) in vitro. see also Figure S1.