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. Author manuscript; available in PMC: 2014 May 6.
Published in final edited form as: Chem Res Toxicol. 2010 Feb 15;23(2):396–404. doi: 10.1021/tx900365u

Figure 1.

Figure 1

Cr(VI) stimulates ISRE-, but not GAS-dependent transactivation. A–B. BEAS-2B cells were exposed to Cr(VI) for indicated times and total RNA was isolated. IRF7 and IRF1 mRNA levels were measured by RT-PCR and real-time PCR, respectively, and normalized against the housekeeping gene, RPL13A. C. BEAS-2B cells were transfected with the ISRE-luc reporter construct. After 24 h, cells were treated with 5 μM Cr(VI) or 100 U/ml IFN-α2 for 4h. Cell lysates were analyzed for luciferase activity. Data are presented as mean ± SEM of fold control (n=3). * and *** designate p<0.05 and p<0.001, respectively.