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. Author manuscript; available in PMC: 2014 May 6.
Published in final edited form as: Chem Res Toxicol. 2010 Feb 15;23(2):396–404. doi: 10.1021/tx900365u

Figure 2.

Figure 2

Cr(VI) induction of IRF7 mRNA requires STAT1. A. Total protein was isolated from BEAS-2B cell lines stably expressing either random (shNC) or STAT1 (shSTAT1) shRNA and total STAT1 and β-actin protein levels were determined by western analysis. B. shNC (open bars) and shSTAT1 (closed bars) cells were exposed to 5 μM Cr(VI) for 0, 1, and 4 h. Total RNA was isolated and IRF7 mRNA was measured by RT-PCR and normalized to the housekeeping gene, RPL13A. C. shNC (open bars) and shSTAT1 (close bars) cells were transiently transfected with the ISRE-luc reporter construct. After 24 h, cells were exposed to 5 μM Cr(VI) for 4 h and luciferase activity was measured. Data are presented as mean ± SEM of fold control (n=3). * and *** designate p<0.05 and p<0.001, respectively, compared to untreated (control) cells. ^ and ^^^ designate p<0.05 and p<0.001, respectively, compared to the shNC cells at the same time point.