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. Author manuscript; available in PMC: 2014 May 6.
Published in final edited form as: Chem Res Toxicol. 2010 Feb 15;23(2):396–404. doi: 10.1021/tx900365u

Figure 6.

Figure 6

Fyn mediates Cr(VI)-activated STAT1 signaling in MEF cells. Wildtype and SYF MEF cells were transiently transfected with either the control vector or the Fyn-myc expression vector. A. Total protein was isolated and Fyn and β-actin protein levels were determined by western analysis. B. Cells were treated with 5 μM Cr(VI) for 2 h and nuclear protein was isolated. pYSTAT1, total STAT1, and β-actin levels were determined by western analysis and a representative blot from a single experiment is shown. C. Density of the protein bands from three separate experiments were quantified with ImageJ software. D. Cells were treated with 5 μM Cr(VI) for 4 h and total RNA was isolated and Irf7 mRNA levels were measured by RT-PCR and normalized to the housekeeping gene, β-actin. Data is presented as mean ± SEM of fold control. * designates p<0.05 compared to respective untreated (control) cells.