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. Author manuscript; available in PMC: 2014 May 6.
Published in final edited form as: Chem Res Toxicol. 2010 Feb 15;23(2):396–404. doi: 10.1021/tx900365u

Figure 7.

Figure 7

Fyn is required for Cr(VI) activation of STAT1 in BEAS-2B cells. A. BEAS-2B cells were exposed to 5 μM Cr(VI) and total Fyn was immunoprecipitated from whole cell lysates and immunoblotted for pFyn and total Fyn. A representative blot from a single experiment is shown. B. Density of the protein bands from three separate experiments were quantified with ImageJ software. C–F. BEAS-2B cells were transduced with GFP-expression control or Fyn shRNA. C. Total protein was isolated and Fyn and β-actin protein levels were determined by western analysis. D. Cells were exposed to 5 μM Cr(VI) for 1 h and nuclear protein was isolated. pYSTAT1, total STAT1, and β-actin levels were determined by western analysis and a representative blot from a single experiment is shown. E. Density of the protein bands from three separate experiments were quantified with ImageJ software. F. Cells were exposed to 5 μM Cr(VI) for 4 h and total RNA was isolated and Irf7 mRNA levels were measured by RT-PCR and normalized to the housekeeping gene, RPL13A. Data is presented as mean ± SEM of fold control. *, **, and *** designate p<0.05, p<0.01, and p<0.001, respectively, compared to respective untreated (control) cells. ^^ designates p<0.01 compared to GFP-expression control-transfected cells.