Table 1.
Primers used for real time qRT-PCR
| Gene | Direction | Sequence | Base pair |
|---|---|---|---|
| HIF-1α | Sense Antisense |
5′-ATTTTTGGACACTGGTGGCT-3′ 5′-ATGCAATGGTGAAATGCTGA-3′ |
200-bp |
| BNIP3 | Sense Antisense |
5′-GCTCCCAGACACCACAAGAT-3′ 5′-TGAGAGTAGCTGTGCGCTTC-3′ |
222-bp |
| β-actin | Sense Antisense |
5′-CCCAACTTGATGTATGAAGG-3′ 5′-TTGTGTAAGGTAAGGTGTGC-3′ |
119-bp |
| GAPDH | Sense Antisense |
5′-TTGTCAAGCTCATTTCCTGGTATG-3′ 5′-GCCATGTAGGCCATGAGGTC-3′ |
76-bp |
BNIP3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; bp, base pair; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HIF-1α, hypoxia inducible factor-1 alpha.