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. 2013 Jul 25;123(8):3564–3576. doi: 10.1172/JCI67521

Figure 7. EGR1 promotes the formation of tendon-like structures from C3H10T1/2 cells.

Figure 7

(A and B) C3H10T1/2 (A) or C3H10T1/2-EGR1 (B) fibrin gel constructs. The 3-week-old tendon-like constructs made with C3H10T1/2-EGR1 cells were larger than those made with C3H10T1/2 cells. (C) Diameters were significantly larger in the presence of Egr1 compared with those of controls. (D) There was no significant increase in cell proliferation based on Ki67+ cell numbers in C3H10T1/2-EGR1 tendon constructs compared with C3H10T1/2 tendon constructs. (E) Longitudinal sections of C3H10T1/2 or C3H10T1/2-EGR1 fibrin gel constructs were immunostained with type I collagen antibody. (F) An increase in the expression levels of Scx, Col1a1, and Col1a2 transcripts was observed in tendon-like constructs from C3H10T1/2-EGR1 cells compared with control constructs from C3H10T1/2 cells. *P < 0.05; ***P < 0.001, unpaired Student’s t tests. Scale bars: 1 mm (A and B); 250 μm (E).