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. 2013 Jul 1;123(8):3317–3330. doi: 10.1172/JCI67930

Figure 5. SCD phenotypic correction.

Figure 5

(A) Phase contrast photomicrographs of deoxygenated erythroid cells. Cells from erythroid differentiation cultures of BM CD34+ cells were treated with sodium metabisulfite, and their morphology was assessed using phase contract microscopy. Five examples of srbc are displayed across the top panels, and 5 examples of nrbc are displayed across the bottom panels. (B) Representative field of rbc from mock-transduced SCD CD34+ cells (left panel) vs. CCL-βAS3-FB transduced SCD CD34+ cells (right panel) upon deoxygenation with sodium metabisulfite. (C) Correlation of the percentage of morphologically “corrected” cells to the VC/cell in each individual culture of CCL-βAS3-FB–transduced SCD BM CD34+ cells. The percentage of corrected rbc is defined as the percentage of nonsickled cells in a transduced sample minus the background value of nonsickled cells in the concordant nontransduced sample.