Table 6.
Sequences of the nine HIV-1 protease cleavage sites within the HIV-1 Gag-Pol polyprotein.
| substrate | P3 | P2 | P1 | P1’ | P2’ | P3’ | P4’ |
|---|---|---|---|---|---|---|---|
| MA/CA | Gln | Asn | Tyr | Pro | Ile | Val | Gln |
| CA/p2* | Arg | Val | Leu | Phe | Glu | Ala | Met |
| p2/NC | Thr | Ile | Met | Met | Gln | Arg | Gly |
| NC/p1 | Gln | Ala | Asn | Phe | Leu | Gly | Lys |
| p1/p6 | Gly | Asn | Phe | Leu | Gln | Ser | Arg |
| TF/PR | Phe | Asn | Phe | Pro | Gln | Ile | Thr |
| PR/RT | Leu | Asn | Phe | Pro | Ile | Ser | Pro |
| RT/RH | Glu | Thr | Phe | Tyr | Val | Asp | Gly |
| RH/IN | Lys | Ile | Leu | Phe | Leu | Asp | Gly |
The cleavage site is between P1 and P1’ residue. The CA/p2 was introduced with an alanine to phenylalanine mutation at P1’ position in order to increase its binding affinity to HIV-1 protease.