Fig. 2.

HRD gains methylation upon introduction of Ssm1b cDNA into mice. (A) The Ssm1b cDNA construct that was introduced into a non-methylating mouse strain (DBA/C3H). (B) Bisulfite analysis: gain of HRD methylation in newborn pups from two independent founder mice with the Ssm1b cDNA transgene. B6 and D2 pups provide controls for complete and low methylation, respectively. (C) Bisulfite analysis showing gain and spread of methylation in the next generation of pups [(founder 1×DBA)F1] carrying the Ssm1b cDNA transgene. (D) Ssm1b gene structure showing exons (1-5) and introns on chromosome 4. Black box, translated sequence; white box, untranslated sequence. (E) Ssm1b protein domains (amino acid boundaries are numbered). NF1-NF3 are non-functional ZFs, whereas ZF1-ZF3 are functional ZFs.