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miRNA target expression is affected in zelda mutants. Wild-type (left) and zelda mutant (right) embryos were hybridized with RNA probes as indicated. Expression of miRNA targets rpr (A), hid (B), abd-A (C), Antp (D) and Ubx (E) is upregulated in zelda mutants. The posterior expansion of Ubx (E) in zelda mutants is partially due to the upregulation of miR-iab-4as (see Fig. 2I), since a similar effect was observed when miR-iab-4as was ectopically expressed (F, right).
