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. 2012 Jun 11;33(7):935–940. doi: 10.1038/aps.2012.46

Figure 3.

Figure 3

Neither SB203580 nor SP600125 prevents anisomycin-induced U251 or U87 cell death. U251 or U87 cells were divided into four groups and separately pre-treated with DMSO vehicle, 10 μmol/L SB203580, 10 μmol/L SP600125, or 10 μmol/L SB203580 plus 10 μmol/L SP600125. After 1 h, the pre-treated cells were treated with 0 or 4 μmol/L anisomycin for 48 h. Then, cell viability was detected using CCK-8 kit. In groups that pre-treated with 10 μmol/L SB203580, 10 μmol/L SP600125, or 10 μmol/L SB203580 plus 10 μmol/L SP600125, anisomycin-induced cell death was not prevented (P>0.05).