Figure 4. Nucleotide dot plot of the PCR amplified genomic region bracketing T. urticae and T. evansi CAS with adjacent intron-containing eukaryotic genes.
The dot-plot was constructed with 95% identity in a 21 bp window, with the T. evansi and the T. urticae amplified region on the y- and x-axis, respectively. From the T. urticae region, the gene models and their genomic positions on the 10th scaffold are specified below the x-axis. The (+) and (−) signs represent the forward and reverse strand, respectively. Blue and black bars indicate exons and introns respectively, while the untranslated regions are depicted as red bars.
Figure 4—figure supplement 1. Agarose gel of PCR products, bracketing CAS with adjacent eukaryotic genes in T. urticae and T. evansi.
λ: lambda DNA, digested with Pstl; A: tetur10g01550—tetur10g01570 fragment in T. urticae; B: tetur10g01570—tetur10g01580 fragment in T. urticae; C; tetur10g01550—tetur10g01570 fragment in T. evansi; D: tetur10g01570—tetur10g01580 fragment in T. evansi. Primers used for the amplification of the fragments are listed in Supplementary file 4.