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. Author manuscript; available in PMC: 2014 May 6.
Published in final edited form as: Hum Immunol. 2013 Jan 17;74(5):574–585. doi: 10.1016/j.humimm.2012.12.017

FIGURE 3.

FIGURE 3

Phosphorylation of T cell signaling proteins in different regulatory T cell subsets from RR and WW donors. Purified T cells from genotyped donors were stimulated by cross-linking of CD3/CD28 for the indicated periods of time, followed by phospho-flow analysis of different regulatory T cell subsets. Data from three to six donor pairs each consisting of one RR and one WW donor were included in each panel. For each pair, data were normalized relative to the phospho-specific signals for the RR naive CD4+ T cells (signals at 0 and 1 min stimulation were set to 0 and 100, respectively). Data are presented as average ± SEM (RR; black, WW; white); *P < 0.05, determined by unpaired t-test.