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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Genes Chromosomes Cancer. 2014 Mar 3;53(6):467–474. doi: 10.1002/gcc.22158

Table 2.

Metaphase FISH Analyses of 14 Myeloma Cell Lines with Three Pairs of IGH Probesa

Cell lineb Normal fusion signalc Primary translocations Secondary or complex IgH rearrangements
#CH,VH signalsd Rearrangements #CH,VH signalsd Rearrangements Rearrangement typee
DP6-DJ 2F 0 0 1CH (0CH) ins CH at 8q24 on der(8)(CH+,VH−,CMYC+,wcp14−) I
U266 2F 0 0 1CH (0CH) ins CH at 11q13 on der(11)t(4;11)(?;q14)(CH+,VH−,CCND1+) I
VP-6 1F 1CH;1VH t(14;16)(q32;q23)(CH+,VH−,CMAF+;CH−,VH+) 2CH (0CH) ins CH at 8q24 on der(8)(CH+,VH−,CMYC+,wcp14−) x2 I
KMS12-BM 2F 1CH;1VH
1VH		 t(11;14)(CH−,VH+;CH+,VH−,CCND1+)f
der(?11)t(?11;14)(CH−,VH+)		 2CH
2CH
1CH
1CH		 ins CH at 8q24 on der(8)(CH+,VH−,CMYC+, wcp14−) x2
der(?)t(?;8)(CH+,VH−,CMYC+,wcp14−) x2
ins CH on der(?) (CH+,VH−,wcp14−) (long chromosome)
ins CH on der(?)(CH+,VH−,wcp14−)(small chromosome)		 I
[I]
I
[I]
JJN3 0 1CH;1VH
1VH		 t(14;16)(q32;q23)(CH+,VH−,CMAF+;CH−,VH+)
der(16)t(14;16)(q32;q23)(CH−,VH+)		 2CH (0CH)
2CH (1CHg)		 ins CH at 8q24 on der(8)(CH+,VH−,CMYC+,wcp14−) x2
der(14)t(8;14;16)(CMYC+,CH+,wcp8+;wcp14+,CHg+,MAF+)		 I
[I+PT]
MM1-144 0 1CH;1VH t(14;16)(q32;q23)(CH+,VH−,CMAF+;CH−,VH+) 1CH (0CH) ins CH at BP on der(3)t(3;8)(?;q24)(CH+,CMYC+) CI
KMS11 0 1CH;1VH
1CH;1VH		 t(4;14)(p16;q32)(CH−,VH+,MMSET+;CH+,VH−,FGFR3+)
t(14;16)(q32;q23)(CH+,VH−,CMAF+;CH−,VH+)		 2CH (0CH) ins CH at BPs on der(?;8;8;?)(wcp14−,CH+,CMYC+,wcp8+, CMYC+,CH+,wcp14−) CI
EJM 1F 1CH
2VH		 der(14)t(7;14;20)(CH+,VH−,MAFB+)
der(20)t(14;20)(q32;q12)(CH−,VH+) x2		 1CH ins CH at BP on der(18)t(7;18)(CH+,VH−,wcp14−) CI
MOLP-8 2F 2CH;2VH t(11;14)(VH+;CH+,VH−,CCND1+)f x2 2CH
2CH		 ins CH on der(4)(wcp14−,CH+,VH−,wcp8−,CMYC+) x2
ins CH on der(17)t(11;17)(q13;?p13)(wcp14−,CH+,VH−,wcp11+) x2		 CI
CI
AMO-1 1F 0 0 2CH (0CH)
2VH
1CH
1CH
2VH		 ins CH at BP on der(8)t(8;12) (CH+,VH−,CMYC+,CCND2+) x2
der(12)(CH−,VH+) x2
der(14)t(8;14)(q24;q32)(CH+,VH−,CMYC+)
der(14)t(8;14)inv(14)(CH+,VH−,CMYC+)
der(?)(CH−,VH+)x2		 CI

T
[T]
OPM-1 0 2CH;2VH der(14)t(4;14)(p16;q32)(CH−,VH+;CH+,VH−)f x2 2CH der(8)t(1;14;8)(CH+,VH−,CMYC+) x2 T
LP-1 0 2CH der(14)t(4;14)(p16;q32)(CH+,VH−,FGFR3+) x2 3F (0CH3VH)
2CH (0CH)
4F (2F2VH)		 der(8)t(3;8)(q24;p23)(CMYC+,wcp14−,CH+,VH+) x3
ins CH on der(?)(wcp14−,CH+,VH−) x2
der(1)t(1;8)(wcp14−,CH+,VH+;wcp8+,CMYC+,wcp14−,CH+,VH+) x2 		VT1A
I
[VT1A]+VT2
KP-6 2F 0 0 1F der(17)(CH+,VH+,wcp14−) VT2
JK-6L 1F 0 0 1F1CH (1CH1VH) t(8;14)(q24;q32)(CH+,VH+;CH+,CMYC+) VT1B
a

In house, Cytocell and Vysis probe sets were used (Fig. 1, Table 1, and text).

b

Cell lines in bold have one or more detection discrepancies among the three probe sets.

c

Normal fusion signals (F) are defined as paired CH/VH signals seen on a normal chromosome 14 or on a derivative chromosome

d

#CH, VH signals is the total number of paired (F) and discrete CH or VH signals observed for different IGH rearrangements. Detection discrepancies (only with the Vysis LSI 3′ Flanking probe) are shown in parentheses, with the bold CH signals identified with the in-house and Cytocell probes but not by the Vysis probe.

e

Type of rearrangement: simple CH insertion (I); complex insertion (CI); non-variant translocation not involving a primary partner (T); primary translocation (PT); variant translocations (VT1a, VT1b, VT2). Brackets indicate rearrangements already accounted for. See text for additional details.

f

Derivative which contains VH signal underwent further rearrangement.

g

CH signal originated from primary translocation.