Figure 8.

Immunohistochemistry analysis of tumor tissues (×132). Paraffin-embedded sections were dewaxed and rehydrated. Endogenous peroxidase activity was quenched by 30% hydrogen peroxide. Antigen retrieval was achieved by microwave treatment. Sections were sequentially incubated with primary antibody, the biotinylated anti-mouse/rabbit antibody, and the streptavidin peroxidase reagent. Peroxidase activity was detected with DAB solution, and sections were weakly counterstained with hematoxylin. PCNA was strongly expressed in PBS-treated mice (A), whereas it was remarkably decreased in ODN1826 (B) or ODN10-treated mice (C). CD63 was strongly expressed in PBS-treated mice (D), whereas it was remarkably decreased in ODN1826 (E) or ODN10-treated mice (F). The tumor stained negatively for co-stimulatory molecules CD80 in PBS-treated mice (G), whereas CD80 was expressed in ODN1826 (H) or ODN10-treated mice (I).