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. 2014 Mar 25;24(5):576–594. doi: 10.1038/cr.2014.33

Figure 3.

Figure 3

MARCKS interaction with Rab10 is important for axon development. (A) The association between Rab10 and MARCKS was blocked by D3 expressed in HEK293 cells. (B, C) Representative images of DIV3 cortical neurons transfected with vehicle plasmid or that encoding MARCKS D1 or D3 fragment (B) and quantification of the axon length (C). Data are shown as mean ± SEM. At least 100 neurons from three independent experiments were analyzed. ***P < 0.001, one-way ANOVA. Scale bar, 20 μm. (D) DIV3 cortical neurons transfected with vehicle plasmid or that encoding D3 fragment were stained with anti-JIP1 and Tuj1 antibodies. Arrows indicate JIP1 signals in axonal tips. Scale bar, 20 μm. (E, F) Representative images of P2 neocortical slices from rat embryos, which were electroporated at E16.5 with vehicle or D3 plasmids, together with pCAG-EYFP (E) and the quantification of corpus callosum axon length (F). Data are presented as mean ± SEM. At least five animals in each group were analyzed. **P < 0.01, Student's t-test. Scale bar, 500 μm.