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. Author manuscript; available in PMC: 2014 May 6.
Published in final edited form as: Nat Protoc. 2012 Sep 20;7(10):1847–1869. doi: 10.1038/nprot.2012.112

Figure 1. A cell free system for centromere and kinetochore reconstitution.

Figure 1

A schematic showing the general experimental procedure and the corresponding steps in the protocol. CENP-A and H3 chromatin arrays are reconstituted from purified proteins and biotinylated high affinity nucleosome positioning sequences using the salt dialysis method. Assembled chromatin arrays are coupled to Streptavidin-coated magnetic beads. The chromatin arrays are incubated in Xenopus egg extracts. After recovery, centromere and kinetochore assembly and function is analyzed. (Figure is adapted from 2).