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. Author manuscript; available in PMC: 2014 Oct 8.
Published in final edited form as: Structure. 2013 Sep 12;21(10):1822–1833. doi: 10.1016/j.str.2013.08.008

Figure 9.

Figure 9

Mechanism of myr-Nef binding to a dDPPG membrane. Myr-Nef is adsorbed through electrostatic attraction and myristate insertion at the first stage. This is identical to the fast process observed by Gerlach et al (Gerlach et al., 2010). At higher surface pressures (35 mN/m), there is no change in the conformation of myr-Nef after the first stage. At lower pressures (20 mN/m) with the insertion of the N-terminal arm into the membrane the (slow process of Gerlach et al) the core domain is displaced 70 A away from the membrane. Surface coverage of myr-Nef in open conformation increases very slowly as a function of time, perhaps involving membrane-driven dimerization (Poe and Smithgall, 2009). Stages I and II occur on timescales that are too fast to be detected by NR or XR.