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Free radical scavenger dimethylsulfoxide, DMSO, affected antibacterial and etching activity of discharge oxygen. One-day old S. aureus biofilms, treated with or without DMSO before they were exposed to discharge oxygen for 25 min. At the end of experiments, live and dead bacteria in biofilms were stained green or red, respectively, by using Live/Dead staining kit and visualized under confocal microscopy.
