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. 2014 Apr 28;20:18. doi: 10.1186/1678-9199-20-18

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Copyright © 2014 Yu et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of HWTX-IV interacting proteins with far-western blotting. Lane 1: without a denaturation/renaturation treatment before incubation with monobiotinylated HWTX-IV. Lane 2: with a denaturation/renaturation treatment before incubation with monobiotinylated HWTX-IV. Lane 3: incubation with HRP-avidin in the absence of monobiotinylated HWTX-IV. P: partial SDS-PAGE image showing the protein band corresponding to the far-western blotting-detected specific band on PVDF membrane. M: partial SDS-PAGE image showing protein molecular weight markers.