Figure 2. Inhibition of AR activity by the engineered repressors.

(a) COS-1 cells were transiently transfected with vectors for the AR, TAT-GRE-E1B-LUC, PDM-LACZ-β-GAL and the repressors or interaction motif either in isolation (solid line) or as a fusion (broken line). (b) PC3-WTAR were transiently transfected with vectors for TAT-GRE-E1B-LUC, PDM-LACZ-β-GAL, AR_1-54 or the repressors. (c) COS-1 cells were transfected with vectors encoding the androgen, glucocorticoid, oestrogen or progesterone receptors (AR, GR, ER, PR), the respective luciferase reporter construct (TAT-GRE-E1B-LUC or ERE-LUC), PDM-LACZ-β-GAL and PLZF_1-452-AR_1-54. Luciferase activity was normalised with β-galactosidase expression and results expressed as a % of AR activity in the absence of the repressors. Mean of 3 independent duplicates ±1SE. T-Test - **p<0.005, ***p<0.0005