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editorial
. 2013 Feb 5;34(2):199–204. doi: 10.1038/aps.2012.202

Table 1. Summary of active antibodies for voltage-sensitive ion channels.

Channel family Antibody Region Epitope sequence Cell type Activities Reference
Voltage-gated potassium channels Anti-Kv 1.2 E3 extracellular loop in domain I FAEADERDSQFPSIP HEK-293 NG108-15 Central medial thalamus (rat) Reduce whole cell current Restore consciousness during anesthesia 16 18
Anti-Kv 3.1 E3 extracellular loop in domain I GAQPNDPSASEHTH HEK-293 NG108-15 Primary oligodendrocyte progenitor cell (mouse) Reduce whole cell current Reduce whole cell current; reduce proliferation and migration 16 26
Anti-Kv 10.1 Fusion of E3 extracellular loop in domain I and tetramerizing coiledcoil GSGSGKWEG HEK-293 Xenopus oocytes Neuroblastoma Breast carcinoma Melanoma Ovarian carcinoma Cervical carcinoma Pancreas carcinoma Colon carcinoma Fibrosarcoma Breast cancer xenograft Pancreatic cancer xenograft Acute myeloid leukemia SH-SY5Y MDA-MB-435s NCI-ADR HT144 C8161 SKMel2 SKOV3 SKOV6 OVCAR-3 OVCAR-8 HeLa BxPC3 HT29 HT1080 MDA-MB-435s Primary PAXF1657 HEL UT-7 K562 PLB-985 Primary cells Reduce whole cell current Reduce tumor growth Reduce proliferation and migration; increase cell death 27 28
Voltagegated sodium channels Anti-Nav (SC-72-14) Extracellular domain N/A Sciatic nerve fibers (rat) Optic nerve fibers (rat) Cardiac purkinje fibers (canine) Sciatic nerve fibers (rat) Reduce whole cell current; reduce action potential amplitude Reduce Vmax; reduce membrane responsiveness Reduce whole cell current; shift the voltage dependence of inactivation 8 11 10
Anti-Nav (SC-72-38) Extracellular domain N/A Myosacs (rat) Sciatic nerve fibers (rat) Shift the voltage-dependence of activation and inactivation Induce channel internalization 12 42
Anti-Nav (SC-66-5) Extracellular domain N/A Sciatic nerve fibers (rat) Optic nerve fibers (rat) Reduce whole cell current 8
Anti-Nav 1.5 E3 extracellular loop in domain I E2 extracellular loop in domain I CVRNFTALNGTNGSVEAD VSENIKLGNLSALRC HEK293 EBNA-293 Reduce whole cell current Reduce whole cell current 17 31
Voltage-gated calcium channels Anti-L-type Extracellular domain N/A BC3H1 myocytes (mouse) Reduce slow current 14
Anti-α1D C-terminal to the pore-forming region between S1 and S2 in domain IV KLCDPDSDYNPGEEYTC Dorsal root ganglion (rat) Cardiac myocytes (guinea-pig) Reduce L-type current (use dependent) 15
Anti-N and P/Q-type P/Q-type E3 extracellular loop DESKEFERDCRGK Cerebellar granule neurons (mouse) HEK293 Purkinje cell soma (mouse) Cerebellum (mouse) Reduce N-type current, P/Q-type current, excitatory postsynaptic current Induce cerebellar ataxia phenotype 19
Anti-P-type E3 extracellular loop IDVEDEDSDEDEFC Small-cell lung carcinoma H146 H209 H345 Reduce P-type current 43
TRP channels Anti-TRPC1 E3 extracellular loop QLYDKGYTSKEQKDC CVGIFCEQQSNDTFHSFIGT Platelets and vascular endothelial cells (human) Vascular smooth muscle cells (human) Bovine aortic endothelial cells Reduce agonist-evoked or store-operated calcium entry Reduce store-operated calcium entry Reduce store-independent, agonist-evoked calcium entry 20,23 21 22
Anti-TRPC5 E3 extracellular loop CYETRAIDEPNNCKG HEK293 CHO Cerebral arterioles (rabbit) Pial arterioles (rabbit) Reduce L-type current 17 24
Anti-TRPM3 E3 extracellular loop CLFPNEEPSWKLAKN HEK293 Reduce whole cell current 25
Anti-TRPV1 E3 extracellular loop EDGKNNSLPMESTPHKC RGSACKP CHO HEK293 Reduce channel activation by proton, heat and chemical ligands 44