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. 2012 Dec 10;34(2):262–270. doi: 10.1038/aps.2012.158

Figure 2.

Figure 2

Chamaejasmenin B and neochamaejasmin C induced DNA damage. (A) A549 cells were exposed to the compounds for 48 h, and protein extracts were immunoblotted for γ-H2AX detection. (B) KHOS cells were exposed to the compounds for 48 h, and protein extracts were immunoblotted for γ-H2AX detection. (C) A549 cells were treated with chamaejasmenin B (2 μmol/L) for 48 h and imaged by immunofluorescence for γ-H2AX (green) and nuclei (blue). γ-H2AX expression is indicated by a punctate appearance in the nucleus. Scale bar=40 μm.