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Effects of [6]-shogaol on protein expression of tyrosinase and MITF. B16 cells were treated with [6]-shogaol at the concentrations indicated above. (A) Tyrosinase expression and (B) MITF expression were analyzed at 48 h and 24 h after treatment, respectively, by Western blotting. Equal protein loading was confirmed using an anti-actin antibody. Con, DMSO-only vehicle control. Data shown are representative of three independent experiments.
