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[6]-shogaol induces activation of ERK, but not of Akt. B16 cells were treated with DMSO only or 10 μmol/L of [6]-shogaol for the indicated time. Whole cell lysates were then subjected to Western blot analysis using antibodies against phosphorylated or total forms of ERK1/2 and Akt. Equal protein loading was confirmed using an anti-actin antibody. C, DMSO only; S, [6]-shogaol. Data shown are representative of three independent experiments.
