Skip to main content
. 2012 Nov 5;34(2):289–294. doi: 10.1038/aps.2012.134

Figure 5.

Figure 5

Suppression of the ERK pathway attenuates [6]-shogaol-induced inhibition of melanin synthesis. B16 cells were pretreated with DMSO only or PD98059 (20 μmol/L) for 1 h, followed by the addition of [6]-shogaol (10 μmol/L). (A) Relative melanin content was measured at 4 d after [6]-shogaol treatment. (B) ERK phosphorylation was examined at 4 h after [6]-shogaol treatment by Western blotting. Equal protein loading was confirmed by actin expression. PD, PD98059. Data are reported as the mean±SEM of three independent experiments carried out in triplicate. cP<0.01 vs control. eP<0.05 vs only PD98059 group.