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. 2012 Dec 31;34(2):231–238. doi: 10.1038/aps.2012.155

Figure 3.

Figure 3

LPS-induced HUVECs apoptosis depends on NHE1, calcium, and calpain. HUVECs were incubated with or without LPS (100 ng/mL) for 24 h in the absence or presence of amiloride (10 μmol/L), BAPTA (0.5 mmol/L), or ZLLal (50 μmol/L). (A) HUVEC cell apoptosis was assayed by TUNEL staining. Scale bar=20 μm. (B) Quantification of TUNEL staining. (C) Detection of DNA fragments. Mean±SEM. n=5. bP<0.05 vs control. eP<0.05 vs LPS alone.