Figure 4.
Amiloride administration suppresses LPS-accelerated atherosclerosis in ApoE−/− mice. ApoE−/− mice were put on a high-fat diet for 6 weeks then treated with or without LPS. Additional cohorts were treated with or without 0.5% amiloride (w/w). (A) HE staining of aortic tissue. Scale bar=100 μm. (B) Bcl-2 protein expression by immunohistochemistry. Scale bar=20 μm. (C) NHE1 activity in mouse aorta extracts. (D) Proposed scheme of LPS-induced endothelial injury and atherosclerosis with amiloride prevention. Mean±SEM. n=5. bP<0.05 vs control. eP<0.05 vs LPS alone.