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. Author manuscript; available in PMC: 2014 May 6.
Published in final edited form as: Leukemia. 2013 Jun 14;27(9):1832–1840. doi: 10.1038/leu.2013.180

Figure 5. Effect of TLR2 shRNA transduction in cultured MDS bone marrow CD34+ cells.

Figure 5

(a) Numbers of myeloid colonies (CFU-G/M) and erythroid colonies (CFU-E) formed in methocult culture two weeks after transduction of TLR2-shRNA and control shRNA in BM CD34+ cells isolated from patients with lower-risk MDS (low-risk and intermediate-1). (b) Representative microphotographs of colonies formed in methocult plates after transduction of TLR2-shRNA (Left panel) and control shRNA (Right panel). Red arrows point to CFU-E. (c) Q-RTPCR analysis of the RNA levels of CD71, EPOR, GYPA and GATA1 in cells collected from total colonies after shRNA transduction and methocult assays.