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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Free Radic Biol Med. 2014 Mar 4;70:214–222. doi: 10.1016/j.freeradbiomed.2014.02.022

Table 1.

Protein S-glutathionylation in the control and 2-AAPA treated H9c2 cells. Protein S-glutathionylation was determined by quantification of GSH released from proteins after NaBH4 reduction as described under “Materials and Methods”. The data are presented as nmol/mg protein and expressed as the means ± SD of three independent experiments.

Time GSH released from proteins (nmol/mg protein)
Control 25 μM 50 μM 75 μM 100 μM
20 min ND* ND* 0.8 ± 0.2 2.7 ± 0.3 4.4 ± 0.6
1 h ND* ND* 1.5 ± 0.4 3.0 ± 0.6 4.7 ± 1.2
2 h ND* ND* ND* 0.9 ± 0.1 1.5 ± 0.3
4 h ND* ND* ND* ND* ND*
*

ND, not detected.