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. 2014 May 6;12(5):e1001856. doi: 10.1371/journal.pbio.1001856

Figure 6. RSF1 is required for the recruitment of CENPS/MHF1, CENPX/MHF2, FANCD2, and FANCI to IRIF.

Figure 6

(A–F) Immunofluorescence of the indicated proteins 60 min after IR (4 Gy) in the indicated siRNA-treated U2OS cells: (A) p-ATM and 53BP1, (B) MHF1 and γ-H2AX, (C) MHF2 and 53BP1, (D) FANCD2 and γ-H2AX, (E) FANCI and γ-H2AX, and (F) FANCD2 and γ-H2AX. (C) Quantification of pATM, γ-H2AX, 53BP1, MHF1, MHF2, FANCI, and FANCD2 IRIF (when cells were depleted of RSF1). At least 100 cells were counted for each set of data; cells with more than 10 foci were considered positive. Error indicates SEM.