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. 2014 May 6;9(5):e95448. doi: 10.1371/journal.pone.0095448

Figure 1. Structure and expression of L2R4 proteins.

Figure 1

Schematic diagram of pFastBac1-E3R4 and pFastBac1-E12R4 constructs (each L2R4 gene is preceded by a signal peptide sequence from baculovirus gp67 for protein secretion) (A). E3R4 or E12R4 consists of three or twelve repeats of HPV16 L2 aa 17–36 and a modified human IgG1 Fc (R4). The R4 is a tandem repeat composed of 4 copies of the hinge and CH2 sequences (HCH2) from IgG fused to an Fc region of IgG (B). The expression of E3R4 and E12R4 in culture medium were performed by reducing SDS-PAGE with Coomassie blue staining (C), and Western blot (D) using RG-1 (1∶5,000). The recombinant proteins were purified by rProtein A affinity column, and the purity was examined by SDS-PAGE with Coomassie blue staining. 10 µg of each L2R4 protein or 12 µg of BSA was loaded (E). Control, Sf9 culture medium; BSA, bovine serum albumin.