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. 2014 May 6;9(5):e95248. doi: 10.1371/journal.pone.0095248

Figure 4. CD8+ T cell activation is sufficient to promote enhanced kinematics.

Figure 4

CTL from OT-1 mice (OT-1), or from B6 mice either immunized with OVA and stimulated with SIINFEKL (Ova-stim), or stimulated with concavalin A (ConA), or not stimulated (Naïve), were added to KC cultures loaded with SIINFEKL and imaged for 30 h. A. CTL were isolated by FACS based on size and granularity and by EGFP and CD8 expression, and stained for CD44. Percentage of isolated cells that are CD44+ are shown. B. Percentage of total KC that were killed after 30 hours of co-culture with different groups of CD8+ T cells. KC killing was determined by red fluorescence change indicating activation of caspase-3. C. Vector displacement and velocity (D) of the effector cells added to antigen-presenting KC over 30 hours of imaging. (A–D N = 5, error bars SEM) (n.s. not significant, *p<0.05; N.S. no significant difference between indicated groups, *p<0.05 between indicated groups ANOVA.).