Figure 3. Effect of Wortmannin on ES uptake in renal cortical slices.

Tissue slices prepared from rat renal cortex were pre-incubated under different experimental conditions as described in the Materials and Methods section. Following pre-incubation, the slices were incubated for 30 min with 50 nM [³H]ES. Values are expressed as means ± SEM from five animals per group (6 slices/animal). **p<0.01, compared to control; ^†† p<0.01, compared to insulin alone.