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. 2014 May 6;9(5):e92475. doi: 10.1371/journal.pone.0092475

Table 2. Calculated values characterizing looping probabilities.

A B C
J P1 4.0 nM 5.13 nM 1.05 nM
J P2 3.6 nM 6.08 nM 1.56 nM
J A1 11.5 nM 6.75 nM 3.59 nM
J A2 16.8 nM 20.51 nM 10.28 nM
K 1 1.00 nM 0.86 nM 0.86 nM
K id 1.47 pM 5.13 pM 5.13 pM
ζ 0.70 0.31 0.57

Calculated values of Inline graphic-factors, and optimized values of binding constants Inline graphic, Inline graphic, and the open loop ratio Inline graphic for the three cases described in the text: A (900 bp DNA with 326 bp loop and 245 nm bead), B (1632 bp DNA with 600 bp loop and 160 nm bead), and C (1632 bp DNA with 900 bp loop and 160 nm bead). The dissociation constants Inline graphic, Inline graphic, and the ratio Inline graphic were obtained by performing an optimized nonlinear fit between the experimental results and theoretical joint distribution as function of [LacI] (see equation (26)) as described in the text. For the case A we used experimental results shown in Figure 3 of [19].