Table 2. Calculated values characterizing looping probabilities.
A | B | C | |
J P1 | 4.0 nM | 5.13 nM | 1.05 nM |
J P2 | 3.6 nM | 6.08 nM | 1.56 nM |
J A1 | 11.5 nM | 6.75 nM | 3.59 nM |
J A2 | 16.8 nM | 20.51 nM | 10.28 nM |
K 1 | 1.00 nM | 0.86 nM | 0.86 nM |
K id | 1.47 pM | 5.13 pM | 5.13 pM |
ζ | 0.70 | 0.31 | 0.57 |
Calculated values of -factors, and optimized values of binding constants , , and the open loop ratio for the three cases described in the text: A (900 bp DNA with 326 bp loop and 245 nm bead), B (1632 bp DNA with 600 bp loop and 160 nm bead), and C (1632 bp DNA with 900 bp loop and 160 nm bead). The dissociation constants , , and the ratio were obtained by performing an optimized nonlinear fit between the experimental results and theoretical joint distribution as function of [LacI] (see equation (26)) as described in the text. For the case A we used experimental results shown in Figure 3 of [19].