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. 2014 May 6;9(5):e96712. doi: 10.1371/journal.pone.0096712

Figure 3. The pH optimum and pH stability of rOs1BGlu4 hydrolysis activity.

Figure 3

A. pH optimum determination: rOs1BGlu4 (0.25 µg) was assayed with 1 mM pNPGlc in different 50 mM pH buffers (formate, pH 4.0; sodium acetate, pH 4.5–5.5; sodium phosphate, pH 6.0–7.5; Tris, pH 8.0–9.5; CAPS, pH 10.0–11.0) at 30°C for 10 min. B. pH stability evaluation: rOs1BGlu4 (20 µg) was incubated in the buffers described above for 10 min, 1, 3, 6, 12 and 24 h, then diluted 40-fold in 50 mM phosphate buffer, pH 6.5, and the activity was determined. The data are provided as mean + SE.