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. 2014 May 6;9(5):e96712. doi: 10.1371/journal.pone.0096712

Figure 5. TLC of hydrolysis products of rOs1BGlu4 with cello-oligosaccharides and laminari-oligosaccharides.

Figure 5

In each 50 µl reaction, 0.125 µg rOs1BGlu4 was incubated with 1 mM oligosaccharide in 50 mM sodium phosphate, pH 6.5, at 30 °C for 20 min. Samples were incubated with (+) and without (−) enzyme. Then, 2 µl of the reaction was spotted onto the TLC plate. Standards and substrates are: G, glucose; C2, cellobiose; C3, cellotriose; C4, cellotetraose; C5, cellopentaose; C6, cellohexaose; L2, laminaribiose; L3, laminaritriose; L4, laminaritetraose and L5, laminaripentaose.