Table 1. Helminth egg recovery obtained with different flotation techniques, tubes and pipettes and detergents.
Method | Number of replicates | Mean number of eggs recovered in 90 µl (Standard deviation) | Mean egg recovery (%) (95% CI) | |
Flotation techniques a | ||||
Mod. McMaster (3 slides) | 5 tubes | 650.3 (90.3) | 64.8 (52.6 – 77.1) | |
FLOTAC basic method | 5 tubes | 433.8 (83.0) | 43.3 (26.4 – 60.1) | |
Tube/pipette material and coating | ||||
Falcon tubes NC and glass pipettes NCb | 5 tubes | 585.6 (168.3) | 58.4 (33.2 – 83.6) | |
Falcon tubes NC and glass pipettes Cc | 5 tubes | 571.2 (170.7) | 57.0 (30.7 – 83.2) | |
Falcon tubes C and glass pipettes C | 3 tubes | 335.2 (120.0) | 33.4 (0 – 74.0) | |
Falcon tubes C and plastic pipettes NC | 3 tubes | 374.1 (162.7) | 37.3 (0 – 86.5) | |
Falcon tubes C and glass pipettes NC | 3 tubes | 355.6 (188.2) | 35.5 (0 – 95.4) | |
Detergent | ||||
Deionized water | 5 bags | 742.2 (56.2) | 74.0 (67.3 – 80.7) | |
7X 1% | 5 bags | 896.6 (228.1) | 89.4 (67.1 – 100) | |
Tween 80 0.1% | 5 bags | 586.6 (180.3) | 58.5 (32.5 – 85.5) | |
Benzethonium chloride 0.1% | 5 bags | 870 (129.2) | 86.7 (73.7 – 99.8) | |
Cetylpyridinium chloride 0.1% | 5 bags | 844.4 (178.1) | 84.2 (65.7 – 100) |
Used with non coated Falcon tubes and plastic pipettes.
NC = non-coated.
C = coated with organosilane.