Skip to main content
. 2014 May 6;9(5):e96731. doi: 10.1371/journal.pone.0096731

Table 1. Helminth egg recovery obtained with different flotation techniques, tubes and pipettes and detergents.

Method Number of replicates Mean number of eggs recovered in 90 µl (Standard deviation) Mean egg recovery (%) (95% CI)
Flotation techniques a
Mod. McMaster (3 slides) 5 tubes 650.3 (90.3) 64.8 (52.6 – 77.1)
FLOTAC basic method 5 tubes 433.8 (83.0) 43.3 (26.4 – 60.1)
Tube/pipette material and coating
Falcon tubes NC and glass pipettes NCb 5 tubes 585.6 (168.3) 58.4 (33.2 – 83.6)
Falcon tubes NC and glass pipettes Cc 5 tubes 571.2 (170.7) 57.0 (30.7 – 83.2)
Falcon tubes C and glass pipettes C 3 tubes 335.2 (120.0) 33.4 (0 – 74.0)
Falcon tubes C and plastic pipettes NC 3 tubes 374.1 (162.7) 37.3 (0 – 86.5)
Falcon tubes C and glass pipettes NC 3 tubes 355.6 (188.2) 35.5 (0 – 95.4)
Detergent
Deionized water 5 bags 742.2 (56.2) 74.0 (67.3 – 80.7)
7X 1% 5 bags 896.6 (228.1) 89.4 (67.1 – 100)
Tween 80 0.1% 5 bags 586.6 (180.3) 58.5 (32.5 – 85.5)
Benzethonium chloride 0.1% 5 bags 870 (129.2) 86.7 (73.7 – 99.8)
Cetylpyridinium chloride 0.1% 5 bags 844.4 (178.1) 84.2 (65.7 – 100)
a

Used with non coated Falcon tubes and plastic pipettes.

b

NC  =  non-coated.

c

C  =  coated with organosilane.