Table 1. Characteristics of commercial α-phosphotyrosine antibodies used in this study.
| Antibody Name | Short namea | Origin/isotype | Used antigen(s) | Given information on antibody specificity | Dilution used | Order number | Company |
| p-Tyr (PY99) | α-PY-99 | mouse-mAB-IgG2b | np | no cross reactivity with Phospho-Ser or Phospho-Thr | 1∶1,000 | sc-7020 | Santa Cruz Biotechnology |
| Anti-phosphotyrosine (clone PY20) | α-PY-20 (BD) | mouse-mAB-IgG2b | np | np | 1∶500 | 61000 | BD Biosciences |
| p-Tyr (PY20) | α-PY-20 (SC) | mouse-mAB-IgG2b | np | np | 1∶1,000 | sc-508 | Santa Cruz Biotechnology |
| Phospho-Tyrosine Mouse mAB (P-Tyr-100) | α-PY-100 | mouse-mAB-IgG1 | mix of phospho-tyrosine peptides (np) | no cross reactivity with Phospho-Ser or Phospho-Thr | 1∶1,000 | 9411 | Cell Signaling |
| Mouse anti-phospho-tyrosine (clone PY69) | α-PY-69 | mouse-mAB-IgG2a | np | np | 1∶1,000 | 610430 | BD Biosciences |
| Phospho-Tyrosine Mouse mAB (P-Tyr-102) | α-PY-102 | mouse-mAB-IgG2 | mix of phospho-tyrosine peptides (np) | no cross reactivity with Phospho-Ser or Phospho-Thr | 1∶1,000 | 9416 | Cell Signaling |
| p-Tyr (PY350) | α-PY-350 | rabbit-pAB-IgG | np | no cross reactivity with Phospho-Ser or Phospho-Thr | 1∶500 | sc-18182 | Santa Cruz Biotechnology |
Abbreviations: IgG (immunoglobulin G); mAB (monoclonal antibody); np (information not provided by the company); pAB (polyclonal antibody); p-Tyr, PY (phosphotyrosine); Ser (serine); Thr (threonine); Tyr (tyrosine).
a
short name used in this study.