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. 2014 May 6;9(5):e96502. doi: 10.1371/journal.pone.0096502

Figure 1. Effect of daphnetin on mouse splenocytes viability and proliferation.

Figure 1

(A) Chemical structure of daphnetin used in the study. (B) Effect of daphnetin on the viability of mouse splenocytes. The cells were treated with daphnetin (0–64 µg/mL) for 48 h. The cell viability was determined by MTT assay. (C) Effect of daphnetin on the ConA induced mouse splenocytes proliferation. Splenocytes cultured with fisetin (4, 8, 16 µg/mL) combined with ConA (5 µg/mL) for 24 h. Cell proliferation was assessed by MTT assay. Data are presented as means ± SD of three independent experiments. Significant differences from control group were indicated by *P<0.05 and **P<0.01.