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. 2014 May 6;9(5):e96533. doi: 10.1371/journal.pone.0096533

Figure 4. Statins promote HO-1 expression by reducing Bach1- and inducing KLF2- expression.

Figure 4

Bach1 (A) and KLF2 (B) expression levels were measured in Huh-5-15 cells by real time RT-qPCR after 6 hours of statin incubation. LucUbiNeo-ET replicon cells were transfected with siRNA directed against KLF2 (siKLF2) or against a control gene (GFP; siControl) at [10 nM] (C, D, E, F). 24 hours after transfection expression of KLF2 and HO-1 was measured by RT-qPCR (C). Cells transfected with siRNA for 24 hours were further incubated with FLV for 24 hours. Expression of KLF2, HO-1 (D) and HCV (E) was measured by RT-qPCR, HCV replication was measured by luciferase reporter assay (F).