Figure 3. TAK1 dependent activation of the MAPK and NFκB pathways in B cells stimulated with combinations of anti-IgG, CPG ODN and BAFF.

Resting tonsill B cells were stimulated with combinations of anti-Ig (2.5 µg/ml), BAFF (100 ng/ml) and CpG (2 µg/ml) for 30 min with or without TAK1 inhibitor ((5Z)-7-Oxozeaenol), and the phosphorylation of various signaling molecules was tested by Western blot.